Authors: Madison S, Wilcox LR.
Title: An evaluation of coronal microleakage in endodontically treated teeth. Part III. In vivo study.
Journal: J Endod
Date: Sep 1988
Citation: 14(9):455-8
Category: Prosthodontic-Endodontic Relationships
Evidence-based Ranking: 5
Purpose/Objectives: To evaluate coronal microleakage of root canal therapy performed in posterior teeth in monkeys.

Materials & Methods: 64 teeth in 4 adult monkeys were used. The canals were chemomechanically prepared with k-Flex files and 2.6% sodium hypochlorite irrigation. After final irrigation and drying of the canals, they were randomly assigned to the experimental groups. Each canal was obturated with laterally condensed gutta-percha and either Roth’s sealer, AH26, or Sealapex. The access openings were closed with Cavit. Positive controls included teeth obturated with gutta-percha and no sealer. Negative controls were obturated with gutta-percha and Roth’s sealer and temporized with IRM. After 72 h the temporary restorations were removed from the experimental and positive control teeth which were then exposed to the oral cavity for 1 wk. After removal of the teeth from the jaw, the teeth were placed in dye and cleared to allow visualization of dye penetration.

Results: AH26 had the highest mean leakage and the Sealapex group the lowest, No significant differences were found between experimental groups and controls.

Author’s Conclusion: The importance of the coronal seal should be emphasized not only during obturation but also when placing interim restorations.

Reviewer’s Comments: The result of this in vivo study did not confirm the finding in the earlier in vitro study. Perhaps the proteins contained in animal saliva were not present in the artificial saliva used in the in vitro study.