Authors: Stanley HR, White CL, McCray L.
Title: The rate of tertiary (reparative) dentine formation in the human tooth.
Journal: Oral Surg Oral Med Oral Pathol
Date: Feb 1966
Citation: 21(2):180-9
Category: Endodontic Biology, Histology and Physiology
Evidence-based Ranking: 4
Purpose/ Objectives: To determine the average daily rate of tertiary dentine formation.

Material and Methods: 108 human teeth were used. Class V cavities had been prepared under local anesthesia and filled with a variety of filling materials, but predominantly with zinc oxide and eugenol. 25 of the specimens were prepared with high-speed water-cooled hand pieces. The postoperative extraction time interval following cavity preparation and filling of tooth ranged from 15 to 132 days. The teeth were then decalcified, sectioned, and mounted. Measurements were made at a magnification of x100.

Results/Summary: Time interval was arbitrarily divided into 3 groups: 27-48 days, 49-71 days and 72-132 days. Little evidence of tertiary dentine formation was observed prior to the thirtieth post-operative day. The rate of formation was highest initially in the 27 to 48 days interval (3.5 u/ day), 49-71 days interval (0.74 u/ day), and 72-132 days interval (0.23 u/day). The average daily rate for the entire study was 1.49 u/day. The milder the operative technique, the longer it takes for the production of tertiary dentine.

ReviewerÂ’s Comments: Even though the study design had flaws (lack of controls, history of previous trauma) it was a decent study demonstrates the different rates of tertiary formation at different intervals.