Authors: Longwill DG, Marshall FJ, Creamer HR.
Title: Reactivity of human lymphocytes to pulp antigens.
Journal: J Endod
Date: Jan 1982
Citation: 8(1):27-32
Category: Pediatric Endodontics
Evidence-based Ranking: 4
Purpose/Objectives: To evaluate whether clinical use of Formocresol induces immune responses in humans to self-antigens or to altered-self-antigens. Materials & Methods: Forty children were included in the study. Group 1 consisted of 20 children, ages 5-9 yrs, with a history of dental treatment that did not include Formocresol pulpotomy; Group 2 consisted of 20 children with a history of 2 or more pulpotomies which were performed 5-18 months prior to taking the blood sample. Blood samples were taken from all individuals and a mononuclear-rich leukocyte cell population was prepared. A pool of dental pulp tissue was obtained from extracted human third molars and a soluble extract was made from the pool. The extract was divided into two portions, one of which was incubated for 7 days in 2% Formocresol, and the other of which was untreated. Each individual’s cell sample was treated with both stimulants, one batch with the untreated pulp extract and another batch with the Formocresol-treated batch. The samples were then tested to see if there was an immune response induced in response to the stimulants.

Results: Analysis of lymphocyte transformation data showed no significant differences between the mean values for Group 1 and Group 2 patients. Twenty-five of the 40 individuals were considered immunologically responsive to either treated or untreated human pulp extracts. Author’s Conclusions: The results show that sensitization to pulp antigens in children is neither effected nor affected by the clinical use of Formocresol pulpotomy.